@TOME V2.3
(Nov 2016)
Ref.
-
-
Doc.
Global output mode :
Sort entries by :
Atome Result
Tools Result
PDB occurence
Core Structure Cluster
Sequence Color type :
Secondary Structure
Hydrophobicity
Disulfide Bonds
TCoffee Identity Score
Uniprot annotation
Core Structure Cluster
Contacts with Ligands
Show alignment :
Atome selection
All alignments
Only Template with Ligand
Column output:
Score:
Atome Rank Value
Tools Score
Hide column
Alignment:
Sequence Identity (%)
Tcoffee Alignment Score
Hide column
3D Common Core:
Tito PKB Score
Verify3D Score
QMean Score
Hide column
Structural Clustering:
Cluster Num
3D-Jury Struct. Score
Hide column
Modeller Result :
Model Rank
QMean Score
Dfire Score
Dope Score
Errat Score
Verify3D Score
Modeller Score
Hide column
Complexes Modeling
Show Ligands of Templates
Complexes Affinity Score
Main Binding Site Identity
Hide column
Templates Information:
Uniprot entry
Cath class
Scop class
Smart class
Prosite class
Pfam class
EC Number
Taxonomy
Experiment Method
Hide column
Sequence & Result Tab:
PDB Template Sequence
Complexes Tab
Values color: [
Good
|
Correct
|
Middling
|
Bad
]
Sequence color :
Mutated
Helix
Strand
Conserved
Query sequence : NPFF1_HUMAN: (2016-01-11 )
MEGEPSQPPNSSWPLSQNGTNTEATPATNLTFSSYYQHTSPVAAMFIVAYALIFLLCMVGNTLVCFIVLKNRHMHTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSGLVQGMSVSASVFTLVAIAVERFRCIVHPFREKLTLRKALVTIAVIWALALLIMCPSAVTLTVTREEHHFMVDARNRSYPLYSCWEAWPEKGMRRVYTTVLFSHIYLAPLALIVVMYARIARKLCQAPGPAPGGEEAADPRASRRRARVVHMLVMVALFFTLSWLPLWALLLLIDYGQLSAPQLHLVTVYAFPFAHWLAFFNSSANPIIYGYFNENFRRGFQAAFRARLCPRPSGSHKEAYSERPGGLLHRRVFVVVRPSDSGLPSESGPSSGAPRPGRLPLRNGRVAHHGLPREGPGCSHLPLTIPAWDI
Atome Classification :
(20 SA)
.........10........20.......---.30.......-----.40..------......50........60--.........70........80........90........100...-.-...110.......120.......130.......140.......150--.--.-----...---...160.......-170.......180.......190.......---200---.---......---210--..--......220.......230.......240.--------.....----.250----------------------.--------------------------------.----------------------------------------------------------------------------------------------------------------------------------------------------.------------------------------------------------------------------------------------------------------------------------------------------------------.----------------...260.----......270.......280....------...290--..---.....----300----........310.......320..---.....330.......340.......350.......360.......370.......380.......390.......400.......410.......420.......430
(Atome)
(Ident)
(Tito)
(Num)
(Ligand)
(Uniprot)
MEGEPSQPPNSSWPLSQNGTNTEATPAT---N
L
T
F
S
S
Y
YQH-----TS
P
V
A
------
A
M
F
I
V
A
Y
A
L
I
F
L
L
C
M
VG---N
T
L
V
C
F
I
V
LKNRHMH
T
V
T
N
M
F
I
L
N
L
A
V
S
D
L
L
V
G
I
FCM
P
T
T
L
V
D
N
L
-I-TGWPFDNA
T
C
K
M
S
G
L
VQGMSV
S
A
S
V
F
T
L
V
A
I
A
V
E
R
F
R
C
I
V
H
P
F
R
-
E
--
K
--
L
-----
T
L
R
---
K
A
L
V
T
I
A
V
I
W
A
L
A
-
L
L
I
MCPS
A
V
T
L
T
V
TREE
H
H
F
M
V
DARNRSY
P
---
L
Y
S
---
C
---
W
E
A
W
P
E
---
K
G
---
M
R
--
R
V
Y
T
T
V
L
F
S
H
I
Y
L
A
P
L
A
L
I
V
V
M
Y
A
R
I
A
R
K
L
--------CQAPG----PAPG----------------------G--------------------------------E----------------------------------------------------------------------------------------------------------------------------------------------------E------------------------------------------------------------------------------------------------------------------------------------------------------A----------------AD
P
R
A
S
R
----
R
R
A
R
V
V
H
M
L
V
M
V
A
L
F
F
T
L
S
W
L
P
L
------
W
A
L
L
L
L
--
I
D---YGQLS----AP-----
Q
L
H
L
V
T
V
Y
A
F
P
F
A
H
W
L
A
F
F
NSSA---N
P
I
I
Y
G
YF
N
E
N
F
R
R
G
F
Q
A
A
F
R
A
RLCPRPSGSH
K
E
A
YSERPGG
L
L
H
R
R
V
F
V
V
V
RPSDSGLPSESGPSSGAPRPGRLPLRNGRVAHHGLPREGPGCSHL
P
L
TIPAWDI
38
HHSearch
92.40
27%
-155
- C3 -
3PBL
2
DRD3_HUMAN (first)
A:
[41-472]
-----------------------------------------------------------
Y
A
L
S
Y
C
A
L
I
L
A
I
V
F
G
---
N
G
L
V
C
M
A
V
L
K
E
R
A
L
Q
T
T
T
N
Y
L
V
V
S
L
A
V
A
D
L
L
V
A
T
L
V
M
P
W
V
V
Y
L
E
V
T
G
-
G
V
W
N
F
S
R
I
C
C
D
V
F
V
T
L
D
V
M
M
C
T
A
S
I
W
N
L
C
A
I
S
I
D
R
Y
T
A
V
V
M
P
V
H
-
Y
--
Q
--
H
G
T
G
Q
S
S
C
R
---
R
V
A
L
M
I
T
A
V
W
V
L
A
-
F
A
V
S
C
P
L
L
F
G
F
N
T
T
G
D
-----------------
P
T
V
---
C
---
S
I
S
N
P
--------------
D
F
V
I
Y
S
S
V
V
S
F
Y
L
P
F
G
V
T
V
L
V
Y
A
R
I
Y
V
V
L
--------
K
Q
R
R
R
----
K
N
I
F
----------------------
E
--------------------------------
M
L
R
I
D
E
GLRL
K
I
Y
K
D
TEG
Y
Y
T
I
G
IGHLLTKSPS
L
N
A
A
K
S
E
L
D
K
A
I
GRNTNGVIT
K
D
E
A
E
K
L
F
N
Q
D
V
D
A
A
V
R
G
I
L
R
N
A
K
L
K
P
V
Y
D
S
LD
A
V
R
R
A
A
L
I
N
M
V
F
Q
MG
E
T
G
V
A
G
F
T
N
S
L
R
M
L
Q
Q
KR
W
D
E
A
A
V
N
L
A
K
S
R
W
Y
N
--------------
Q
------------------------------------------------------------------------------------------------------------------------------------------------------
T
P
N
R
A
K
R
V
I
T
T
F
R
T
GTW
D
A
Y
G
V
P
L
----
R
E
K
K
A
T
Q
M
V
A
I
V
L
G
A
F
I
V
C
W
L
P
F
------
F
L
T
H
V
L
--
N
T
---
H
C
Q
T
C
-----------
H
---
V
S
P
E
L
Y
S
A
T
T
W
L
G
Y
V
N
S
A
L
---
N
P
V
I
Y
T
T
F
N
I
E
F
R
K
A
F
L
K
I
L
S
C
------------------------------------------------------------------------------------
32
HHSearch
90.64
24%
-158
- C3 -
3RZE
5
ENLYS_BPT4 (first)
A:
[10-443]
----------------------------------------------------------
P
L
V
V
V
L
S
T
I
C
L
V
T
V
G
L
---
N
L
L
V
L
Y
A
V
R
S
E
R
K
L
H
T
V
G
N
L
Y
I
V
S
L
S
V
A
D
L
I
V
G
A
V
V
M
P
M
N
I
L
Y
L
L
-
M
-
S
K
W
S
L
G
R
P
L
C
L
F
W
L
S
M
D
Y
V
A
S
T
A
S
I
F
S
V
F
I
L
C
I
D
R
Y
R
S
V
Q
Q
P
L
R
-
Y
LK
Y
--
R
-----
T
K
T
---
R
A
S
A
T
I
L
G
A
W
F
L
S
-
F
L
W
V
I
P
I
L
G
-
W
N
H
---------------
R
R
---
E
D
K
---
C
---
E
T
D
F
Y
D
---
V
--------
T
W
F
K
V
M
T
A
I
I
N
F
Y
L
P
T
L
L
M
L
W
F
Y
A
K
I
Y
K
A
V
--------
R
Q
H
C
N
----
I
F
E
M
----------------------
L
R
I
D
E
G
L
R
L
K
I
Y
K
D
TEG
Y
Y
T
I
G
IGHLLTKSPS
L
N
A
A
K
S
E
L
D
K
A
I
GRNTNGVIT
K
D
E
A
E
K
L
F
N
Q
D
V
D
A
A
V
R
G
I
L
R
NAKL
K
P
V
Y
D
S
LD
A
V
R
R
A
A
L
I
N
M
V
F
Q
MG
E
T
G
V
A
G
F
T
N
S
L
R
M
L
Q
Q
KR
W
D
E
A
A
V
N
L
A
K
S
R
W
Y
N
Q
T----------------------------------------------
P
------------------------------------------------------------------------------------------------------------------------------------------------------
N
R
A
K
R
V
I
T
T
F
R
TGTW--
D
A
Y
L
H
M
N
----
R
E
R
K
A
A
K
Q
L
G
F
I
M
A
A
F
I
L
C
W
I
P
Y
------
F
I
F
F
M
V
--
I
A
---
F
C
K
N
C
----
C
-----------
N
E
H
L
H
M
F
T
I
W
L
G
Y
I
N
S
T
L
---
N
P
L
I
Y
P
L
C
N
E
N
F
K
K
T
F
K
R
I
L
H
I
------------------------------------------------------------------------------------
33
HHSearch
89.07
26%
-158
- C3 -
2RH1
16
ADRB2_HUMAN (first)
A:
[41-477]
---------------------------------------------------------
V
G
M
G
I
V
M
S
L
I
V
L
A
I
V
F
G
---
N
V
L
V
I
T
A
I
A
K
F
E
R
L
Q
T
V
T
N
Y
F
I
T
S
L
A
C
A
D
L
V
M
G
L
A
V
V
P
F
G
A
A
H
I
L
-
M
-
K
M
W
T
F
G
N
F
W
C
E
F
W
T
S
I
D
V
L
C
V
T
A
S
I
E
T
L
C
V
I
A
V
D
R
Y
F
A
I
T
S
P
F
K
-
Y
--
Q
SL
L
-----
T
K
N
---
K
A
R
V
I
I
L
M
V
W
I
V
S
-
G
L
T
S
F
L
P
I
Q
M
H
W
Y
R
A
T
H
Q
E
----
A
I
N
-
C
Y
A
---
E
E
T
---
C
---
C
D
F
F
T
----------
N
--
Q
A
Y
A
I
A
S
S
I
V
S
F
Y
V
P
L
V
I
M
V
F
V
Y
S
R
V
F
Q
E
A
--------
K
R
Q
L
N
----
I
F
E
M
----------------------
L
--------------------------------
R
I
D
E
G
L
R
L
K
I
Y
K
D
TEGYYTIGIGHLLTKSPS
L
N
A
A
K
S
E
L
D
K
A
I
GRNTNGVIT
K
D
E
A
E
K
L
F
N
Q
D
V
D
A
A
V
R
G
I
L
R
N
A
K
L
K
P
V
Y
D
S
LD
A
V
R
R
A
A
L
I
N
M
V
F
Q
MG
E
T
G
V
A
G
F
T
N
S
L
R
M
L
Q
Q
KR
W
D
E
A
A
V
N
L
A
K
S
R
W
Y
N
Q
T--------------
P
N
R
A
K
R
V
I
T
T
F
R
TGT----------------------------------------------------------------------------------------------------------------------------------------
W
----------------
D
A
Y
K
F
C
L
----
K
E
H
K
A
L
K
T
L
G
I
I
M
G
T
F
T
L
C
W
L
P
F
------
F
I
V
N
I
V
--
H
V
---
I
Q
D
N
L
---------------
I
R
K
E
V
Y
I
L
L
N
W
I
G
Y
V
N
S
G
F
---
N
P
L
I
Y
C
-
R
S
P
D
F
R
I
A
F
Q
E
L
L
C
L
------------------------------------------------------------------------------------
30
HHSearch
87.44
22%
-149
- C3 -
3UON
4
ACM2_HUMAN (first)
A:
[26-457]
-----------------------------------------------------------
I
V
L
V
A
G
S
L
S
L
V
T
I
I
G
---
N
I
L
V
M
V
S
I
K
V
N
R
H
L
Q
T
V
N
N
Y
F
L
F
S
L
A
C
A
D
L
I
I
G
V
F
S
M
N
L
Y
T
L
Y
T
V
-
I
-
G
Y
W
P
L
G
P
V
V
C
D
L
W
L
A
L
D
Y
V
V
S
N
A
S
V
M
N
L
L
I
I
S
F
D
R
Y
F
C
V
T
K
P
L
T
-
Y
--
P
VK
R
-----
T
T
K
---
M
A
G
M
M
I
A
A
A
W
V
L
S
-
F
I
L
W
A
P
A
I
L
F
W
Q
F
I
V
G
V
-----
R
----
T
V
E
---
D
G
E
---
C
---
Y
I
Q
F
F
S
---------
N
--
A
A
V
T
F
G
T
A
I
A
A
F
Y
L
P
V
I
I
M
T
V
L
Y
W
H
I
S
R
A
S
--------
K
S
R
I
N
----
I
F
E
M
----------------------
L
--------------------------------
R
I
D
EGLRL
K
I
Y
K
D
TEG
Y
Y
T
I
GIGHLLTKSPS
L
N
A
A
K
S
E
L
D
K
A
I
GRNTNGVIT
K
D
-----------------------------------------------------------------------------------------------
E
A
E
K
L
F
N
Q
D
V
D
A
A
V
R
G
I
L
R
NAKL
K
P
V
Y
D
S
LD
A
V
R
R
A
A
L
I
N
M
V
F
Q
MG
E
T
G
V
A
G
F
T
N
S
L
R
M
L
Q
Q
KR
W
D
E
A
A
V
N
L
A
K
S
R
W
Y
N
Q
-----------------------------------------------------------------------
T
P
N
R
A
K
R
V
I
T
T
F
R
TGTW
D
A
Y
P
P
P
S
----
R
E
K
K
V
T
R
T
I
L
A
I
L
L
A
F
I
I
T
W
A
P
Y
------
N
V
M
V
L
I
--
N
T
---
F
C
A
P
C
---------------
I
P
N
T
V
W
T
I
G
Y
W
L
C
Y
I
N
S
T
I
---
N
P
A
C
Y
A
L
C
N
A
T
F
K
K
T
F
K
H
L
L
M
-------------------------------------------------------------------------------------
36
HHSearch
87.09
23%
-150
- C3 -
3EML
13
ENLYS_BPT4
A:
[22-470]
---------------------------------------------------------
S
V
Y
I
T
V
E
L
A
I
A
V
L
A
I
L
G
---
N
V
L
V
C
W
A
V
W
L
N
S
N
L
Q
N
V
T
N
Y
F
V
V
S
L
A
A
A
D
I
A
V
G
V
L
A
I
P
F
A
I
T
I
S
----
T
G
F
C
A
A
C
H
G
C
L
F
I
A
C
F
V
L
V
L
T
Q
S
S
I
F
S
L
L
A
I
A
I
D
R
Y
I
A
I
R
I
P
L
R
-
Y
N
G
L
--
V
-----
T
G
T
---
R
A
K
G
I
I
A
I
C
W
V
L
S
-
F
A
I
G
L
T
P
M
L
G
W
N
N
C
G
Q
-------
S
Q
G
C
G
E
G
---
Q
V
A
---
C
---
L
F
E
D
V
V
--------
P
M
--
N
Y
M
V
Y
F
N
F
F
A
C
V
L
V
P
L
L
L
M
L
G
V
Y
L
R
I
F
L
A
A
--------
R
R
Q
L
N
----
I
F
E
M
----------------------
L
--------------------------------
R
I
D
E
GLRL
K
I
Y
K
D
TEGYYTIGIGHLLTKSPS
L
N
A
A
K
S
E
L
D
K
A
I
GRNTNGVIT
K
D
E
A
E
K
L
F
N
Q
D
V
D
A
A
V
R
G
I
L
R
N
A
K
L
K
P
V
Y
D
S
LD
A
V
R
R
A
A
L
I
N
M
V
F
Q
MG
E
T
G
V
A
G
F
T
N
S
L
R
M
L
Q
Q
KR
W
D
E
A
A
V
N
L
A
K
S
R
W
Y
N
Q
T--------------
P
N
R
A
K
R
V
I
T
T
F
R
TGTW---------------------------------------------------------------------------------------------------------------------------------------
D
----------------
A
Y
R
S
T
L
Q
----
K
E
V
H
A
A
K
S
L
A
I
I
V
G
L
F
A
L
C
W
L
P
L
------
H
I
I
N
C
F
--
T
F
---
F
C
P
D
C
-----
S
--------
H
A
P
L
W
L
M
Y
L
A
I
V
L
S
H
T
N
S
V
V
---
N
P
F
I
Y
A
Y
R
I
R
E
F
R
Q
T
F
R
K
I
I
R
S
H
V
L
R
Q
-------------------------------------------------------------------------------
39
HHSearch
83.37
25%
-151
- C3 -
3V2Y
1
ENLYS_BPT4
A:
[63-495]
--------------------------------------------------
I
------
K
L
T
S
V
V
F
I
L
I
C
C
F
I
I
L
E
---
N
I
F
V
L
L
T
I
W
K
T
K
K
F
H
R
P
M
Y
Y
F
I
G
N
L
A
L
S
D
L
L
A
G
V
A
Y
T
A
N
L
L
L
S
G
--
A
-
T
T
Y
K
L
T
P
A
Q
W
F
L
R
E
G
S
M
F
V
A
L
S
A
S
V
F
S
L
L
A
I
A
I
E
R
Y
I
T
M
L
K
----------------
N
N
F
---
R
L
F
L
L
I
S
A
C
W
V
I
S
-
L
I
L
G
G
L
P
I
M
G
W
N
C
I
S
--------------
A
---
L
S
S
---
C
---
S
T
V
L
P
L
---
Y
-----
H
--
K
H
Y
I
-
L
F
C
T
T
V
F
T
L
L
L
L
S
I
V
I
L
Y
C
R
I
Y
S
L
V
--------
R
T
R
N
I
----
F
E
M
L
----------------------
R
--------------------------------
I
DEGLRL
K
I
Y
K
D
TE
G
Y
Y
T
I
G
IGHLLTKSPS
L
N
A
A
K
S
E
L
D
K
A
I
GRNTNGVIT
K
D
E
A
E
K
L
F
N
Q
D
V
D
A
A
V
R
G
I
L
R
N
A
K
L
K
P
V
Y
D
S
LD
A
V
R
R
A
A
L
I
N
M
V
F
Q
MG
E
T
G
V
A
G
F
T
N
S
L
R
M
L
Q
Q
KR
W
D
E
A
A
V
N
L
A
K
S
R
W
Y
N
Q
T
P
N
R
A
K
R
V
I
T
T
F
R
TGT
W
------------------------------------------------------------------------------------------------------------------------------------------------------
D
----------------
A
Y
A
S
R
S
S
----
E
N
V
A
L
L
K
T
V
I
I
V
L
S
V
F
I
A
C
W
A
P
L
------
F
I
L
L
L
L
--
D
V
---
G
C
K
V
K
----
T
C
---------
D
I
L
F
R
A
E
Y
F
L
V
L
A
V
L
N
S
G
T
---
N
P
I
I
Y
T
L
T
N
K
E
M
R
R
A
F
I
R
I
M
G
R
P
L
----------------------------------------------------------------------------------
51
Fugue
80.18
21%
-158
- C3 -
4XNV
15
P2RY1_HUMAN (first)
A:
[38-382]
---------------------------------
S
F
K
C
A
L
T
K
-----
T
G
F
Q
F
------
Y
Y
L
P
A
V
Y
I
L
V
F
I
I
G
F
L
G
---
N
S
V
A
I
W
M
F
V
F
H
M
K
P
W
S
G
I
S
V
Y
M
F
N
L
A
L
A
D
F
L
Y
-
V
L
T
L
P
A
L
I
F
Y
Y
F
-
N
K
T
D
W
I
F
G
D
A
M
C
K
L
Q
R
F
I
F
H
V
N
L
Y
G
S
I
L
F
L
T
C
I
S
A
H
R
Y
S
G
V
V
Y
P
K
S
L
G
--
R
--
L
-----
K
K
K
---
N
A
I
C
I
S
V
L
V
W
L
I
V
-
V
V
A
I
S
P
-----------
I
L
F
Y
S
G
T
G
V
R
K
N
K
---
T
I
T
---
C
---
Y
D
T
T
S
D
---
E
Y
---
L
R
S
Y
F
I
Y
S
M
C
T
T
V
A
M
F
C
V
P
L
V
L
I
L
G
C
Y
G
L
I
V
R
A
L
I
Y
K
M
K
K
Y
T
C
T
V
C
G
Y
I
Y
N
P
E
D
G
DPDNGVNPGTDFKDIVCPLCGV
G
--------------------------------
K
----------------------------------------------------------------------------------------------------------------------------------------------------
D
------------------------------------------------------------------------------------------------------------------------------------------------------
Q
----------------
F
E
E
V
E
E
P
----
L
R
R
K
S
I
Y
L
V
I
I
V
L
T
V
F
A
V
S
Y
I
P
F
------
H
V
M
K
T
M
--
N
L
---
R
A
R
L
D
----
F
Q
TPAM
C
A
F
N
D
R
V
Y
A
T
Y
Q
V
T
R
G
L
A
S
L
N
S
C
V
---
N
P
I
L
Y
F
L
A
G
D
T
F
R
R
R
-------------------------------------------------------------------------------------------
5
PsiBlast_PDB
80.16
27%
-132
- C3 -
2KSB
1
NK1R_HUMAN
A:
[38-345]
--------------------------------------------------------------
A
Y
T
V
I
V
V
T
S
V
V
G
---
N
V
V
V
M
W
I
I
L
A
H
K
R
M
R
T
V
T
N
Y
F
L
V
N
L
A
F
A
E
A
S
M
A
A
F
N
T
V
V
N
F
T
Y
A
V
-
H
-
N
E
W
Y
Y
G
L
F
Y
C
K
F
H
N
F
F
P
I
A
A
V
F
A
S
I
Y
S
M
T
A
V
A
F
D
R
Y
M
A
I
I
H
P
L
Q
-
P
--
R
--
L
-----
S
A
T
---
A
T
K
V
V
I
C
V
I
W
V
L
A
-
L
L
L
A
F
P
Q
G
Y
Y
S
T
T
E
T
M
P
S
R
V
V
------------------
C
---
M
I
E
W
P
E
HPN
K
I
---
Y
E
--
K
V
Y
H
I
C
V
T
V
L
I
Y
F
L
P
L
L
V
I
G
Y
A
Y
T
V
V
G
I
T
L
--------
W
A
S
E
I
----
P
G
D
S
----------------------
S
--------------------------------
D
----------------------------------------------------------------------------------------------------------------------------------------------------
R
------------------------------------------------------------------------------------------------------------------------------------------------------
Y
----------------
H
E
Q
V
S
A
K
----
R
K
V
V
K
M
M
I
V
V
V
C
T
--
F
A
I
C
W
L
P
F
------
H
I
F
F
L
L
P
Y
I
N
PDL
Y
L
K
K
F
----
I
Q
-----
Q
V
Y
L
A
I
M
-------
W
L
A
M
S
S
T
M
Y
---
N
P
I
I
Y
C
C
L
N
D
R
F
R
L
G
F
K
H
A
F
R
C
--
C
P
F
I
S
A
G
D
Y
E
G
L
E
M
K
S
T
R
Y
L
Q
T
Q
G
S
V
Y
K
V
S
R
L
E
T
T
I
S
T
V
V
G
A
----------------------------------------
4
PsiBlast_PDB
80.16
27%
-132
- C3 -
2KSA
1
NK1R_HUMAN
A:
[38-345]
--------------------------------------------------------------
A
Y
T
V
I
V
V
T
S
V
V
G
---
N
V
V
V
M
W
I
I
L
A
H
K
R
M
R
T
V
T
N
Y
F
L
V
N
L
A
F
A
E
A
S
M
A
A
F
N
T
V
V
N
F
T
Y
A
V
-
H
-
N
E
W
Y
Y
G
L
F
Y
C
K
F
H
N
F
F
P
I
A
A
V
F
A
S
I
Y
S
M
T
A
V
A
F
D
R
Y
M
A
I
I
H
P
L
Q
-
P
--
R
--
L
-----
S
A
T
---
A
T
K
V
V
I
C
V
I
W
V
L
A
-
L
L
L
A
F
P
Q
G
Y
Y
S
T
T
E
T
M
P
S
R
V
V
------------------
C
---
M
I
E
W
P
E
HPN
K
I
---
Y
E
--
K
V
Y
H
I
C
V
T
V
L
I
Y
F
L
P
L
L
V
I
G
Y
A
Y
T
V
V
G
I
T
L
--------
W
A
S
E
I
----
P
G
D
S
----------------------
S
--------------------------------
D
----------------------------------------------------------------------------------------------------------------------------------------------------
R
------------------------------------------------------------------------------------------------------------------------------------------------------
Y
----------------
H
E
Q
V
S
A
K
----
R
K
V
V
K
M
M
I
V
V
V
C
T
--
F
A
I
C
W
L
P
F
------
H
I
F
F
L
L
P
Y
I
N
PDL
Y
L
K
K
F
----
I
Q
-----
Q
V
Y
L
A
I
M
-------
W
L
A
M
S
S
T
M
Y
---
N
P
I
I
Y
C
C
L
N
D
R
F
R
L
G
F
K
H
A
F
R
C
--
C
P
F
I
S
A
G
D
Y
E
G
L
E
M
K
S
T
R
Y
L
Q
T
Q
G
S
V
Y
K
V
S
R
L
E
T
T
I
S
T
V
V
G
A
----------------------------------------
3
PsiBlast_PDB
80.16
27%
-132
- C3 -
2KS9
1
NK1R_HUMAN
A:
[38-345]
--------------------------------------------------------------
A
Y
T
V
I
V
V
T
S
V
V
G
---
N
V
V
V
M
W
I
I
L
A
H
K
R
M
R
T
V
T
N
Y
F
L
V
N
L
A
F
A
E
A
S
M
A
A
F
N
T
V
V
N
F
T
Y
A
V
-
H
-
N
E
W
Y
Y
G
L
F
Y
C
K
F
H
N
F
F
P
I
A
A
V
F
A
S
I
Y
S
M
T
A
V
A
F
D
R
Y
M
A
I
I
H
P
L
Q
-
P
--
R
--
L
-----
S
A
T
---
A
T
K
V
V
I
C
V
I
W
V
L
A
-
L
L
L
A
F
P
Q
G
Y
Y
S
T
T
E
T
M
P
S
R
V
V
------------------
C
---
M
I
E
W
P
E
HPN
K
I
---
Y
E
--
K
V
Y
H
I
C
V
T
V
L
I
Y
F
L
P
L
L
V
I
G
Y
A
Y
T
V
V
G
I
T
L
--------
W
A
S
E
I
----
P
G
D
S
----------------------
S
--------------------------------
D
----------------------------------------------------------------------------------------------------------------------------------------------------
R
------------------------------------------------------------------------------------------------------------------------------------------------------
Y
----------------
H
E
Q
V
S
A
K
----
R
K
V
V
K
M
M
I
V
V
V
C
T
--
F
A
I
C
W
L
P
F
------
H
I
F
F
L
L
P
Y
I
N
PDL
Y
L
K
K
F
----
I
Q
-----
Q
V
Y
L
A
I
M
-------
W
L
A
M
S
S
T
M
Y
---
N
P
I
I
Y
C
C
L
N
D
R
F
R
L
G
F
K
H
A
F
R
C
--
C
P
F
I
S
A
G
D
Y
E
G
L
E
M
K
S
T
R
Y
L
Q
T
Q
G
S
V
Y
K
V
S
R
L
E
T
T
I
S
T
V
V
G
A
----------------------------------------
50
Fugue
79.48
23%
-108
- C3 -
1U19
15
OPSD_BOVIN
A:
[4-349]
M
N
G
T
E
--
G
P
N
F
Y
V
P
F
S
----
N
K
T
G
V
V
R
S
---
P
F
E
A
P
Q
Y
Y
L
A
-----
E
P
W
Q
F
------
S
M
L
A
A
Y
M
F
L
L
I
M
L
G
F
P
I
---
N
F
L
T
L
Y
V
T
V
Q
H
K
K
L
R
T
P
L
N
Y
I
L
L
N
L
A
V
A
D
L
F
M
V
F
G
G
F
T
T
T
L
Y
T
S
L
-
H
-
G
Y
F
V
F
G
P
T
G
C
N
L
E
G
F
F
A
T
L
G
G
E
I
A
L
W
S
L
V
V
L
A
I
E
R
Y
V
V
V
C
K
P
M
S
-
N
F
-
R
--
F
-----
G
E
N
---
H
A
I
M
G
V
A
F
T
W
V
M
A
-
L
A
C
A
A
P
P
L
V
G
W
S
--------------
R
Y
I
P
E
GM
Q
C
S
---
C
G
I
D
Y
Y
T
P
H
E
---
E
T
---
N
N
--
E
S
F
V
I
Y
M
F
V
V
H
F
I
I
P
L
I
V
I
F
F
C
Y
G
Q
L
V
F
T
V
-----------------
K
E
A
A
----------------------
A
--------------------------------
Q
----------------------------------------------------------------------------------------------------------------------------------------------------
Q
------------------------------------------------------------------------------------------------------------------------------------------------------
Q
----------------
E
S
A
T
T
Q
K
----
A
E
K
E
V
T
R
M
V
I
I
M
V
I
A
F
L
I
C
W
L
P
Y
------
A
G
V
A
F
Y
--
I
F
---
T
H
Q
G
S
----
D
-------------
F
G
P
I
F
M
T
I
P
A
F
F
A
K
T
S
AV
Y
N
P
V
I
Y
I
M
M
N
K
Q
F
R
N
C
M
V
T
T
L
---
C
C
G
K
N
P
L
G
D
D
E
A
S
T
T
---------------------
V
S
K
T
E
T
S
Q
V
A
P
A
-----------------------------------
53
Fugue
79.24
23%
-121
- C3 -
1F88
8
OPSD_BOVIN
A:
[1-348]
M
N
G
T
E
G
P
N
------
F
Y
V
P
F
S
N
K
T
G
V
V
R
S
---
P
F
E
A
P
Q
Y
Y
L
A
-----
E
P
W
Q
F
------
S
M
L
A
A
Y
M
F
L
L
I
M
L
G
F
P
I
---
N
F
L
T
L
Y
V
T
V
Q
H
K
K
L
R
T
P
L
N
Y
I
L
L
N
L
A
V
A
D
L
F
M
V
F
G
G
F
T
T
T
L
Y
T
S
L
-
H
-
G
Y
F
V
F
G
P
T
G
C
N
L
E
G
F
F
A
T
L
G
G
E
I
A
L
W
S
L
V
V
L
A
I
E
R
Y
V
V
V
C
K
P
M
S
-
N
F
-
R
--
F
-----
G
E
N
---
H
A
I
M
G
V
A
F
T
W
V
M
A
-
L
A
C
A
A
P
P
L
V
G
W
S
--------------
R
Y
I
P
E
GM
Q
C
S
---
C
G
I
D
Y
Y
T
P
H
E
---
E
T
---
N
N
--
E
S
F
V
I
Y
M
F
V
V
H
F
I
I
P
L
I
V
I
F
F
C
Y
G
Q
L
V
F
T
V
-------------------------------------------
K
--------------------------------
E
----------------------------------------------------------------------------------------------------------------------------------------------------
A
------------------------------------------------------------------------------------------------------------------------------------------------------
A
----------------
A
S
A
T
T
Q
K
----
A
E
K
E
V
T
R
M
V
I
I
M
V
I
A
F
L
I
C
W
L
P
Y
------
A
G
V
A
F
Y
--
I
F
---
T
H
Q
G
S
----
D
F
-----
G
P
I
F
M
T
I
P
A
F
-----
F
A
K
T
S
A
V
Y
---
N
P
V
I
Y
I
M
M
N
K
Q
F
R
N
C
M
V
T
T
L
C
C
G
------------------------------
K
N
P
S
T
T
V
S
K
T
E
T
S
Q
V
A
P
A
-----------------------------------
15
PsiBlast_PDB
79.00
27%
-133
- C3 -
4N6H
23
C562_ECOLX (first)
A:
[78-414]
---------
D
D
A
L
K
L
A
N
E
G
K
V
K
E
A
Q
A
A
A
E
Q
L
K
T
T
R
N
A
Y
I
Q
K
YL---
G
S
P
G
A
R
S
A
S
S
L
A
L
A
I
A
I
T
A
L
Y
S
A
V
C
A
V
G
L
L
G
N
V
L
V
M
F
G
I
V
R
Y
T
K
M
K
T
A
T
N
I
Y
I
F
N
L
A
L
A
D
A
L
-
A
T
S
T
L
P
F
Q
S
A
K
Y
L
-
M
-
E
T
W
P
F
G
E
L
L
C
K
A
V
L
S
I
D
Y
Y
N
M
F
T
S
I
F
T
L
T
M
M
S
V
D
R
Y
I
A
V
C
H
P
V
K
-
A
-----
L
-----
D
F
R
T
P
A
K
A
K
L
I
N
I
C
I
W
V
L
A
-
S
G
V
G
V
P
I
M
V
-
M
A
V
T
R
P
R
D
G
A
V
V
C
M
L
Q
F
P
S
P
---
S
W
Y
-------
W
D
T
----------------
V
T
K
I
C
V
F
L
F
A
F
V
V
P
I
L
I
I
T
V
C
Y
G
L
M
L
L
R
L
--------
R
S
V
R
L
----
L
S
G
S
----------------------
K
--------------------------------
E
----------------------------------------------------------------------------------------------------------------------------------------------------
K
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------
D
R
----
S
L
R
R
I
T
R
M
V
L
V
V
V
G
A
F
V
V
C
W
A
P
I
H
I
F
V
I
V
W
T
L
V
D
I
--
D
R
---
R
D
P
L
V
----
V
A
-----
A
L
H
L
C
I
A
--------
L
G
Y
A
N
S
S
L
---
N
P
V
L
Y
A
F
L
D
E
N
F
K
R
C
F
R
-----
Q
L
C
R
K
P
C
G
----------------------------------------------------------------------------
17
PsiBlast_PDB
78.64
26%
-160
- C3 -
2Y00
13
ADRB1_MELGA
A:
[19-296]
----------------------------------------------------------------
A
L
V
V
L
L
I
V
A
G
---
N
V
L
V
I
A
A
I
G
S
T
Q
R
L
Q
T
L
T
N
L
F
I
T
S
L
A
C
A
D
L
V
V
G
L
L
V
V
P
F
G
A
T
L
V
V
-
R
-
G
T
W
L
W
G
S
F
L
C
E
L
W
T
S
L
D
V
L
C
V
T
A
S
I
E
T
L
C
V
I
A
I
D
R
Y
L
A
I
T
S
P
F
R
-
Y
--
Q
S
L
M
-----
T
R
A
---
R
A
K
V
I
I
C
T
V
W
A
I
S
-
A
L
V
--------
S
F
L
P
I
M
M
H
W
W
R
D
E
D
P
Q
A
L
K
---
C
Y
Q
DPG
C
-------
C
D
---
F
V
---
T
N
--
R
A
Y
A
I
A
S
S
I
I
S
F
Y
I
P
L
L
I
M
I
F
V
A
L
R
V
Y
R
-----------
E
A
K
E
----
Q
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
S
R
V
M
L
M
R
E
H
K
A
L
K
T
L
G
I
I
M
G
V
F
T
L
C
W
L
P
F
------
F
L
V
N
I
V
--
N
V
---
F
N
R
---------------
D
L
V
P
D
W
L
F
V
A
F
N
W
L
G
Y
A
N
S
A
M
---
N
P
I
I
Y
C
R
-
S
P
D
F
R
K
A
F
K
R
L
L
A
-------------------------------------------------------------------------------------
13
PsiBlast_PDB
78.63
26%
-135
- C3 -
4RWA
2
C562_ECOLX (first)
A:
[78-406]
---------
D
D
A
L
K
L
A
N
E
G
K
V
K
E
A
Q
A
A
A
E
Q
L
K
T
T
R
N
A
Y
I
Q
K
YLGAR
S
A
S
S
L
------
A
L
A
I
A
I
T
A
L
Y
S
A
V
C
A
V
G
L
L
G
N
V
L
V
M
F
G
I
V
R
Y
T
K
M
K
T
A
T
N
I
Y
I
F
N
L
A
L
A
D
A
L
-
A
T
S
T
L
P
F
Q
S
A
K
Y
L
-
M
-
E
T
W
P
F
G
E
L
L
C
K
A
V
L
S
I
D
Y
Y
N
M
F
T
S
I
F
T
L
T
M
M
S
V
D
R
Y
I
A
V
C
H
P
V
K
-
A
-----
L
-----
D
F
R
T
P
A
K
A
K
L
I
N
I
C
I
W
V
L
A
-
S
G
V
G
V
P
I
M
V
-
M
A
V
T
R
P
R
D
G
A
V
V
C
M
L
Q
F
P
S
P
---
S
W
Y
-------
W
D
T
----------------
V
T
K
I
C
V
F
L
F
A
F
V
V
P
I
L
I
I
T
V
C
Y
G
L
M
L
L
---------------------
G
S
----------------------
K
--------------------------------
E
----------------------------------------------------------------------------------------------------------------------------------------------------
K
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------
D
R
----
S
L
R
R
I
T
R
M
V
L
V
V
V
G
A
F
V
V
C
W
A
P
I
H
I
F
V
I
V
W
T
L
V
D
I
--
D
R
---
R
D
P
L
V
----
V
A
-----
A
L
H
L
C
I
A
--------
L
G
Y
A
N
S
S
L
---
N
P
V
L
Y
A
F
L
D
E
N
F
K
R
C
F
R
-----
Q
L
C
---------------------------------------------------------------------------------
16
PsiBlast_PDB
78.54
26%
-156
- C3 -
4GBR
1
ADRB2_HUMAN
A:
[10-286]
-------------------------------------------------------------
I
V
M
S
L
I
V
L
A
I
V
F
G
---
N
V
L
V
I
T
A
I
A
K
F
E
R
L
Q
T
V
T
N
Y
F
I
T
S
L
A
C
A
D
L
V
M
G
L
A
V
V
P
F
G
A
A
H
I
L
-
T
-
K
T
W
T
F
G
N
F
W
C
E
F
W
T
S
I
D
V
L
C
V
T
A
S
I
E
T
L
C
V
I
A
V
D
R
Y
F
A
I
T
S
P
F
K
-
Y
--
Q
SL
L
-----
T
K
N
---
K
A
R
V
I
I
L
M
V
W
I
V
S
G
L
T
S
F
L
P
--
I
Q
M
H
W
Y
R
A
T
H
Q
E
A
I
N
C
----
Y
A
---
E
E
T
---
C
---
C
D
F
F
T
N
---
Q
A
---
Y
A
--
I
A
S
S
I
V
S
F
----
Y
V
P
L
V
I
M
V
F
V
Y
S
R
V
F
Q
E
-------------------
A
K
R
----------------------
Q
--------------------------------
L
----------------------------------------------------------------------------------------------------------------------------------------------------
Q
------------------------------------------------------------------------------------------------------------------------------------------------------
K
----------------
I
D
-
K
F
C
L
----
K
E
H
K
A
L
K
T
L
G
I
I
M
G
T
F
T
L
C
W
L
P
F
------
F
I
V
N
I
-----------
V
H
----
V
I
-----
Q
D
N
L
I
R
K
E
V
Y
I
L
L
N
W
I
G
Y
V
N
S
G
F
---
N
P
L
I
Y
C
R
-----
S
P
D
F
R
I
A
F
Q
E
L
L
C
L
--------------------------------------------------------------------------------
14
PsiBlast_PDB
78.46
26%
-131
- C3 -
4RWD
7
C562_ECOLX (first)
A:
[78-406]
---------
D
D
A
L
K
L
A
N
E
G
K
V
K
E
A
Q
A
A
A
E
Q
L
K
T
T
R
N
A
Y
I
Q
K
YLGAR
S
A
S
S
L
------
A
L
A
I
A
I
T
A
L
Y
S
A
V
C
A
V
G
L
L
G
N
V
L
V
M
F
G
I
V
R
Y
T
K
M
K
T
A
T
N
I
Y
I
F
N
L
A
L
A
D
A
L
-
A
T
S
T
L
P
F
Q
S
A
K
Y
L
-
M
-
E
T
W
P
F
G
E
L
L
C
K
A
V
L
S
I
D
Y
Y
N
M
F
T
S
I
F
T
L
T
M
M
S
V
D
R
Y
I
A
V
C
H
P
V
K
-
A
-----
L
-----
D
F
R
T
P
A
K
A
K
L
I
N
I
C
I
W
V
L
A
-
S
G
V
G
V
P
I
M
V
-
M
A
V
T
R
P
R
D
G
A
V
V
C
M
L
Q
F
P
S
P
---
S
W
Y
-------
W
D
T
----------------
V
T
K
I
C
V
F
L
F
A
F
V
V
P
I
L
I
I
T
V
C
Y
G
L
M
L
L
R
L
--------
R
S
V
R
L
----
L
S
G
S
----------------------
K
--------------------------------
E
----------------------------------------------------------------------------------------------------------------------------------------------------
K
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------
D
R
----
S
L
R
R
I
T
R
M
V
L
V
V
V
G
A
F
V
V
C
W
A
P
I
H
I
F
V
I
V
W
T
L
V
D
I
--
D
R
---
R
D
P
L
V
----
V
A
-----
A
L
H
L
C
I
A
--------
L
G
Y
A
N
S
S
L
---
N
P
V
L
Y
A
F
L
D
E
N
F
K
R
C
F
R
-----
Q
L
C
---------------------------------------------------------------------------------
34
HHSearch
78.03
29%
-154
- C3 -
2KS9
1
NK1R_HUMAN
A:
[34-322]
----------------------------------------------------------
L
W
A
A
A
Y
T
V
I
V
V
T
S
V
V
G
---
N
V
V
V
M
W
I
I
L
A
H
K
R
M
R
T
V
T
N
Y
F
L
V
N
L
A
F
A
E
A
S
M
A
A
F
N
T
V
V
N
F
T
Y
A
V
-
H
-
N
E
W
Y
Y
G
L
F
Y
C
K
F
H
N
F
F
P
I
A
A
V
F
A
S
I
Y
S
M
T
A
V
A
F
D
R
Y
M
A
I
I
H
P
L
Q
-
P
--
R
--
L
-----
S
A
T
---
A
T
K
V
V
I
C
V
I
W
V
L
A
-
L
L
L
A
F
P
Q
G
Y
Y
S
T
T
E
T
M
P
------------
S
---
R
V
V
---
C
---
M
I
E
W
P
E
---
H
P
NKI
Y
E
--
K
V
Y
H
I
C
V
T
V
L
I
Y
F
L
P
L
L
V
I
G
Y
A
Y
T
V
V
G
I
T
L
--------
W
A
S
E
I
----
P
G
D
S
----------------------
S
--------------------------------
D
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
R
Y
H
E
Q
V
S
----
A
K
R
K
V
V
K
M
M
I
V
V
V
C
T
F
A
I
C
W
L
P
F
------
H
I
F
F
L
L
--
P
Y
---
I
N
P
D
L
----
Y
-------
L
K
K
F
I
Q
Q
V
Y
L
A
I
M
W
L
A
M
S
S
T
M
Y
---
N
P
I
I
Y
C
C
L
N
D
R
F
R
L
G
F
K
H
A
F
R
C
C
P
F
I
S
A
G
D
Y
E
G
L
E
M
K
S
T
R
Y
L
Q
T
Q
G
S
V
Y
K
V
S
R
L
E
T
T
I
S
T
V
V
G
A
------------------------------------------
31
HHSearch
77.75
23%
-138
- C3 -
3ODU
6
CXCR4_HUMAN (first)
A:
[47-496]
-------------------------------------------------
N
K
------
I
F
L
P
T
I
Y
S
I
I
F
L
T
G
I
V
G
---
N
G
L
V
I
L
V
M
G
Y
Q
K
K
L
R
S
M
T
D
K
Y
R
L
H
L
S
V
A
D
L
L
F
V
-
I
T
L
P
F
W
A
V
D
A
V
---
A
N
W
Y
F
G
N
F
L
C
K
A
V
H
V
I
Y
T
V
N
L
Y
S
S
V
W
I
L
A
F
I
S
L
D
R
Y
L
A
I
V
H
A
T
N
-
S
--
Q
R
P
R
-----
K
L
L
---
A
E
K
V
V
Y
V
G
V
W
I
P
A
-
L
L
L
T
I
P
D
F
I
F
A
N
V
S
E
A
D
------------
D
---
R
Y
I
---
C
---
D
R
F
Y
P
N
---
D
L
---
W
V
--
V
V
F
Q
F
Q
H
I
M
V
G
L
I
L
P
G
I
V
I
L
S
C
Y
C
I
I
I
S
K
L
--------
S
H
S
G
S
----
N
I
F
E
----------------------
M
--------------------------------
L
----------------------------------------------------------------------------------------------------------------------------------------------------
R
I
D
E
G
L
R
L
K
I
Y
K
D
TEG
Y
Y
T
I
GIGHLLTKSPS
L
N
A
A
K
S
E
L
D
K
A
IGRNTNGVIT
K
D
E
A
E
K
L
F
N
Q
D
V
D
A
A
V
R
G
I
L
R
NAKLK
P
V
Y
D
S
LD
A
V
R
R
A
A
L
I
N
M
V
F
Q
MG
E
T
G
V
A
G
F
T
N
S
L
R
M
L
Q
Q
KRW
D
E
A
A
V
N
L
A
K
S
R
W
Y
N
Q
T
P
N
R
A
K
R
V
I
T
T
F
R
TGTW
D
----------------
A
Y
G
S
K
G
H
----
Q
K
R
K
A
L
K
T
T
V
I
L
I
L
A
F
F
A
C
W
L
P
Y
------
Y
I
G
I
S
I
--
D
S
---
F
I
L
L
E
I
I
K
Q
G
C
-----
E
F
E
N
T
V
H
K
W
I
S
I
T
E
A
L
A
F
F
H
C
C
L
---
N
P
I
L
Y
A
F
L
G
A
K
F
K
T
S
A
Q
H
A
L
T
S
G
R
P
L
E
V
L
F
Q
---------------------------------------------------------------------------
18
PsiBlast_PDB
77.39
26%
-157
- C3 -
2Y01
12
ADRB1_MELGA
A:
[19-296]
----------------------------------------------------------------
A
L
V
V
L
L
I
V
A
G
---
N
V
L
V
I
A
A
I
G
S
T
Q
R
L
Q
T
L
T
N
L
F
I
T
S
L
A
C
A
D
L
V
V
G
L
L
V
V
P
F
G
A
T
L
V
V
-
R
-
G
T
W
L
W
G
S
F
L
C
E
L
W
T
S
L
D
V
L
C
V
T
A
S
I
E
T
L
C
V
I
A
I
D
R
Y
L
A
I
T
S
P
F
R
-
Y
--
Q
S
L
M
-----
T
R
A
---
R
A
K
V
I
I
C
T
V
W
A
I
S
-
A
L
V
--------
S
F
L
P
I
M
M
H
W
W
R
D
E
D
P
Q
A
L
K
---
C
Y
Q
D
PG
C
-------
C
D
---
F
V
---
T
N
--
R
A
Y
A
I
A
S
S
I
I
S
F
Y
I
P
L
L
I
M
I
F
V
A
L
R
V
Y
R
-----------
E
A
K
E
----
Q
--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
S
R
V
M
L
M
R
E
H
K
A
L
K
T
L
G
I
I
M
G
V
F
T
L
C
W
L
P
F
------
F
L
V
N
I
V
--
N
V
---
F
N
R
---------------
D
L
V
P
D
W
L
F
V
A
F
N
W
L
G
Y
A
N
S
A
M
---
N
P
I
I
Y
C
R
-
S
P
D
F
R
K
A
F
K
R
L
L
A
-------------------------------------------------------------------------------------